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Last update on 04. Mar 2022 .
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    Design Sequencing Primers

    OCCURRENCE  

    ARB_NT/Probes/Design Sequencing Primers

     

    DESCRIPTION  

    This module will search for primers for all positions.

    The best result is one primer for all (marked) taxa, the worst case are n primers for n taxa.

    • does only search in marked sequences.
    • sequences need to be aligned.

    Searches for sections in alignment which require the lowest number of primer combinations. You have to specify the maximum allowed number of different primers. Specify '1' to report only primers valid for all marked species.

    The module outputs a list of primer candidates, probably outputs hundreds of pages. Checking these primers requires a lot of manual work.

    Press ctrl-C to abort the program.

     

    Field experience  

    Bad sequences confuse the algorithm. Workaround: unmark them.

     

    NOTES  

    None

     

    EXAMPLES  

    None

     

    WARNINGS  

    None

     

    BUGS  

    No bugs known