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Last update on 25. Nov 2018 .
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    Calculate sequence quality

    OCCURRENCE

    ARB_NT/Sequence/Calculate sequence quality

     

    DESCRIPTION

    'Calculate sequence quality' tries to measure the quality of sequences and the quality their alignment.

    HANDLING:

    Fill in the values you think are appropriate. The default values are the values that worked best in the first test runs. Many criteria are evaluated (see 'THE VALUES' below for details).
    A final "quality-value" (percentage) for each sequence is calculated and all sequences below the given threshold may get marked.

    HOW IT WORKS:

    In the section "weights" you have quite a few options to fill in.
    These are some of the criteria used to evaluate the quality of the sequences.. The values represent the share of the criteria in the final evaluation-formula. All values represent percentages, therefore all values together should sum up to 100.

    THE VALUES:

    Base analysis:
    This is the number of bases that are stored in the sequence. "-" and "." are not counted.
    Deviation:
    This is the deviation of the number of bases from a sequence to the average number of bases in a group.
    No Helices:
    This is the number of positions in a sequence where no helix structure can be built.
    Consensus:
    For each named group found in the tree (selected below) a consensus sequence is calculated.
    Every species' sequence is compared against the consensus sequences of all groups of which the species is a member.
    That comparison uses conformity with and deviation from the consensus sequence.
    IUPAC:
    This is the number of iupac-codes stored in a sequence.
    GC proportion:
    This is the deviation in GC proportion from a sequence to group.
     

    NOTES

    Generally speaking the consensus is the mightiest tool to evaluate the quality. So keep the percentage high unless you know what you're doing or you want to evaluate with just one or two values.

    Be aware that the computation is very complex and can easily take hours to finish. So if you don't see the statusbar moving in the first ten minutes it just means that you are analyzing a huge database.

     

    EXAMPLES

    None

     

    WARNINGS

    None

     

    BUGS

    No bugs known