By checking the 'Save settings' toggle, the used values for the start position and the translation table are written into the corresponding fields ('codon_start' and 'transl_table') of every "translated species". That happens in Manual and Automatic mode.
By checking the 'Translate all data' button, the translation insert a 'X' in front of the generated amino acid sequence, if you select starting position 2 or 3 and if there are nucleotides in front of that starting position. Later automatic realignments would fail if that 'X' is missing!
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