The SAI entry 'ECOLI' is used by the editor and other programs to detect and display the current cursor position with respect to the homologous position within the E. coli sequence.
If you have no SAI 'ECOLI' yet this creates one:
Search your ecoli sequence and select it.
Go to species information window and
press SPECIES/COPY to create a copy of the ´Selected species´. This will create a species with ID 'xxx0' or similar, where 'xxx' is the ID of your selected species.
select the 'name' field and change it to 'ECOLI'
press 'SPECIES/Convert to SAI'
NOTES
After inserting or deleting gaps in the whole alignment (ARB_NT/ Sequence/Insert/Delete column) the reference SAI has to be reloaded by this function.
You may as well choose any other sequence as reference sequence.